Bruserud O, Pawelec G
Second Department of Internal Medicine, Tübingen University Medical Clinic, F.R.G.
Int J Immunopharmacol. 1993 Feb;15(2):93-7. doi: 10.1016/0192-0561(93)90085-d.
Because Cyclosporine A (CsA) is often reported to inhibit only the proliferation of quiescent T-cells and to have much less or no effect on activated proliferating T-cells, the effects of CsA and FK506 on long-term in vitro T-cell proliferation were investigated for alloreactive T-cell clones and clones derived from a leukaemia patient early after allogeneic bone marrow transplantation. Drug effects were tested in the presence of exogenous IL2 or IL2 + IL4 during weekly T-cell activation/restimulation. In the presence of either drug, cloned T-cells were able to respond to a reduced number of restimulations (mitogenic activation, allostimulation) and could undergo fewer cell divisions/population doublings. These effects were seen in the presence of both excess IL2 or IL2 + IL4.
由于经常有报道称环孢素A（CsA）仅抑制静止T细胞的增殖，而对活化增殖的T细胞作用很小或没有作用，因此研究了CsA和FK506对同种异体反应性T细胞克隆以及同种异体骨髓移植后早期从一名白血病患者分离出的克隆的长期体外T细胞增殖的影响。在每周的T细胞激活/再刺激过程中，在外源性IL2或IL2 + IL4存在的情况下测试药物效果。在任何一种药物存在的情况下，克隆的T细胞对减少数量的再刺激（促有丝分裂激活、同种异体刺激）都有反应，并且能够经历较少的细胞分裂/群体倍增。在过量IL2或IL2 + IL4存在的情况下都观察到了这些效果。
