Serke S, Huhn D
Abteilung Innere Medizin und Poliklinik, Universitätsklinikum Rudolf Virchow, Freie Universität, Berlin.
Clin Lab Haematol. 1993;15(1):33-44. doi: 10.1111/j.1365-2257.1993.tb00119.x.
Applying thiazole orange stain and multiparameter flow-cytometry, immature erythrocytes, e.g., reticulocytes, can be discriminated from the mature erythrocytes by virtue of their higher RNA-content. One major problem of this method, however, consists in the inclusion of nucleated cells (leucocytes) and large RNA-containing platelets in the population defined by light scatter-patterns as erythrocytes. Due to the high intensity of staining with thiazole orange of all these cellular elements, all of them in the analysis are prone to be classified erroneously as reticulocytes. In order to classify elements in analysis properly as erythroid (reticulocytes+mature erythrocytes) or as non-erythroid (leucocytes+platelets), an alternative staining method is shown in this paper, consisting of thiazole orange combined to anti-glycophorin-A-PE-monoclonal antibody.
应用噻唑橙染色和多参数流式细胞术，未成熟红细胞，如网织红细胞，可因其较高的RNA含量与成熟红细胞区分开来。然而，该方法的一个主要问题在于，在通过光散射模式定义为红细胞的群体中包含有核细胞（白细胞）和含大量RNA的血小板。由于所有这些细胞成分对噻唑橙的染色强度很高，在分析中它们都容易被错误地分类为网织红细胞。为了将分析中的成分正确分类为红系（网织红细胞 + 成熟红细胞）或非红系（白细胞 + 血小板），本文展示了一种替代染色方法，即噻唑橙与抗血型糖蛋白A-藻红蛋白单克隆抗体联合使用。
