Scarabelli L, Giannoni P, Malfatto C, Bolognesi C, Cesarone C F
Institute of General Physiology, University of Genoa, Italy.
Mutat Res. 1993 May;302(1):1-6. doi: 10.1016/0165-7992(93)90082-7.
The genotoxic effects due to in vivo treatment with zinc dithiocarbamates were evaluated in rat and mouse liver. The two pesticides Zineb and Ziram, belonging to this chemical class, induced an increase in single-strand DNA breaks, as measured by the alkaline elution technique. The nuclear enzyme poly(ADP-ribose) polymerase (pADPRP), a chromatin-bound catalytic protein, utilizing NAD+ as a substrate, was tested by a radiometric procedure. A close relationship between the increased extent of DNA damage and the enhanced level of endogenous pADPRP activity was obtained in rat liver, whereas both parameters remained unchanged in mouse liver.
在大鼠和小鼠肝脏中评估了二硫代氨基甲酸锌体内处理所产生的遗传毒性效应。属于该化学类别的两种农药代森锌和福美锌，通过碱性洗脱技术测定，可诱导单链DNA断裂增加。利用NAD⁺作为底物的核酶聚（ADP - 核糖）聚合酶（pADPRP），一种与染色质结合的催化蛋白，通过放射性测定法进行检测。在大鼠肝脏中，DNA损伤程度的增加与内源性pADPRP活性水平的提高之间存在密切关系，而在小鼠肝脏中这两个参数均保持不变。
