Roque R S, Caldwell R B
Department of Anatomy & Cell Biology, Texas College of Osteopathic Medicine, Fort Worth 76107-2699.
Curr Eye Res. 1993 Mar;12(3):285-90. doi: 10.3109/02713689308999475.
In the Royal College of Surgeons rat, the migration of phagocytic cells into the subretinal space accompanies photoreceptor cell death during the early stages of retinal dystrophy. These are followed closely by cellular alterations in the retinal pigment epithelium, Müller cells, and outer retinal vessels. We have identified the phagocytic cells as microglia and hypothesized that they may be involved in the above cellular changes. Thus, we developed procedures for their isolation and growth. Our study shows that retina-derived microglia (1) are positive for microglial markers Griffonia simplicifolia isolectin B4, Mac-1 alpha, phosphotyrosine, and vimentin; (2) are highly phagocytic; and (3) respond to macrophage colony stimulating factor by proliferating. This culture system would provide a valuable tool in studying mechanisms of cellular alterations in retinal disease.
在皇家外科学院大鼠中，在视网膜营养不良的早期阶段，吞噬细胞向视网膜下间隙的迁移伴随着光感受器细胞死亡。随后，视网膜色素上皮、Müller细胞和视网膜外层血管会出现细胞改变。我们已确定这些吞噬细胞为小胶质细胞，并推测它们可能参与了上述细胞变化。因此，我们开发了分离和培养它们的方法。我们的研究表明，源自视网膜的小胶质细胞：（1）对小胶质细胞标志物四叶豆凝集素B4、Mac-1α、磷酸酪氨酸和波形蛋白呈阳性；（2）具有高度吞噬性；（3）对巨噬细胞集落刺激因子有增殖反应。这种培养系统将为研究视网膜疾病中细胞改变的机制提供一个有价值的工具。
