Royce L S, Kibbey M C, Mertz P, Kleinman H K, Baum B J
Clinical Investigations and Patient Care Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
Differentiation. 1993 Mar;52(3):247-55. doi: 10.1111/j.1432-0436.1993.tb00637.x.
Culture of the human neoplastic submandibular gland intercalated duct cell line, HSG, on the basement membrane extract Matrigel induces dramatic morphologic changes and cytodifferentiation. Transmission electron microscopy demonstrated an acinar cell phenotype with polarized cells containing a well-developed Golgi apparatus, multiple microvilli-like projections from the apical surfaces into a lumenal-like area, and numerous granule-like organelles. Amylase, an acinar cell marker, was detected by both immunocytochemical and Northern blot analyses. A 50% reduction in [3H]thymidine incorporation by cells cultured on Matrigel, as compared to cells cultured on tissue culture plates, confirmed the differentiated phenotype of the cells. Multiple components of Matrigel appear to contribute to the morphologic differentiation of the HSG cells since antibodies to both laminin and collagen IV, as well as the laminin-derived bioactive peptide containing SIKVAV, have potent inhibitory effects on HSG cell organization on Matrigel. Collectively, these data indicate that culture of HSG cells on Matrigel is a useful model to study salivary gland acinar development.
将人肿瘤性下颌下腺闰管细胞系HSG培养于基底膜提取物基质胶上，可诱导显著的形态学变化和细胞分化。透射电子显微镜显示出腺泡细胞表型，细胞呈极化状态，含有发育良好的高尔基体、从顶端表面伸向类似管腔区域的多个微绒毛样突起以及众多颗粒样细胞器。通过免疫细胞化学和Northern印迹分析均检测到腺泡细胞标志物淀粉酶。与在组织培养板上培养的细胞相比，在基质胶上培养的细胞中[3H]胸苷掺入量减少50%，证实了细胞的分化表型。基质胶的多种成分似乎都对HSG细胞的形态学分化有贡献，因为针对层粘连蛋白和IV型胶原的抗体，以及含有SIKVAV的层粘连蛋白衍生生物活性肽，对HSG细胞在基质胶上的组织化均有强大的抑制作用。总体而言，这些数据表明在基质胶上培养HSG细胞是研究唾液腺腺泡发育的有用模型。
