Stolt P, Zillig W
Max-Planck-Institut für Biochemie, Martinsried, Germany.
Mol Microbiol. 1993 Mar;7(6):875-82. doi: 10.1111/j.1365-2958.1993.tb01178.x.
Strains of the extremely halophilic archaebacterium Halobacterium salinarium that are lysogenic for the phage phi H produce an antisense RNA transcript complementary to the first 151 nucleotides (nt) of the early lytic phage transcript T1. This is the first case of antisense control of gene expression in an archaebacterium. We show through transformation of H. salinarium that the antisense RNA functions in trans, rendering the early lytic phage transcript T1 susceptible to specific cleavage by an unidentified RNase of unique endonucleolytic activity. The single-stranded ends of RNA are cut off at the ends of the 151 nt RNA duplex, removing the ribosomal binding sites from the first open reading frame of transcript T1 but without concomitant digestion of the products.
对噬菌体φH呈溶原性的极端嗜盐古细菌盐沼盐杆菌菌株产生一种反义RNA转录本，它与早期裂解噬菌体转录本T1的前151个核苷酸（nt）互补。这是古细菌中基因表达反义控制的首例。我们通过盐沼盐杆菌的转化实验表明，反义RNA在反式作用中发挥功能，使早期裂解噬菌体转录本T1易于被一种具有独特内切核酸酶活性的未知核糖核酸酶进行特异性切割。RNA的单链末端在151 nt RNA双链体的末端被切断，从转录本T1的第一个开放阅读框中去除核糖体结合位点，但不会同时消化产物。
