Lima M, Portnoi D, Bandeira A, Arala Chaves M
Department of Immunology, Institute for Biomedical Sciences Abel Salazar, Porto, Portugal.
Scand J Immunol. 1993 May;37(5):605-14. doi: 10.1111/j.1365-3083.1993.tb02579.x.
In order to further understand the mechanism mediating the mitogenic and immunosuppressor effects of p90, a protein produced by Streptococcus intermedius, flow cytometric studies were performed on peripheral and central lymphoid organs of mice treated with this protein. p90 induced a strong blastogenic B-cell response in the spleen and lymph nodes, followed by a slight but significant polyclonal T-cell activation. B-cell repertoire analysis indicated that polyclonal B-cell responses affected similarly both CD5+ and conventional (CD5-) B cells in the spleen. Repertoire analysis of T cells failed to reveal any preferential stimulation of the V beta T-cell receptor (V beta-TcR) families studied. Peripheral lymphoid hyperplasia was observed concomitantly with central lymphoid depletion. In the bone marrow, pre-B and B cells were profoundly depleted, with a more pronounced effect on small pre-B cells. In the thymus, double-positive (CD4+CD8+) thymocytes were preferentially eliminated, with a relative enrichment of single positive (either CD4+ or CD8+) and double-negative (CD4-CD8-) thymocytes.
为了进一步了解介导中间型链球菌产生的一种蛋白质p90的促有丝分裂和免疫抑制作用的机制，对用该蛋白质处理的小鼠的外周和中枢淋巴器官进行了流式细胞术研究。p90在脾脏和淋巴结中诱导了强烈的B细胞增殖反应，随后是轻微但显著的多克隆T细胞激活。B细胞谱系分析表明，多克隆B细胞反应对脾脏中的CD5 +和常规（CD5 -）B细胞的影响相似。T细胞的谱系分析未能揭示所研究的VβT细胞受体（Vβ-TcR）家族有任何优先刺激。外周淋巴组织增生与中枢淋巴组织耗竭同时出现。在骨髓中，前B细胞和B细胞被深度耗竭，对小前B细胞的影响更明显。在胸腺中，双阳性（CD4 + CD8 +）胸腺细胞被优先清除，单阳性（CD4 +或CD8 +）和双阴性（CD4 - CD8 -）胸腺细胞相对富集。
