Qureshi S A, Joseph C K, Hendrickson M, Song J, Gupta R, Bruder J, Rapp U, Foster D A
Institute for Biomolecular Structure and Function, Hunter College, City University of New York, New York 10021.
Biochem Biophys Res Commun. 1993 Apr 30;192(2):969-75. doi: 10.1006/bbrc.1993.1510.
A vector expressing a dominant negative mutant of Raf-1 was stably introduced into BALB/c 3T3 cells expressing a temperature-sensitive derivative of v-Src. High levels of the Raf-mutant were detected in these cells. Expression of the Raf-1 mutant blocked v-Src-induced transformation, as determined by reversion to a flat non-transformed morphology and the inability to form colonies in soft agar. Cells transfected with the parental vector lacking the mutant Raf-1 could be transformed by v-Src. These data suggest that intracellular signals activated by v-Src that are mediated by Raf-1 are required for transformation by v-Src.
将表达Raf-1显性负性突变体的载体稳定导入表达v-Src温度敏感衍生物的BALB/c 3T3细胞中。在这些细胞中检测到高水平的Raf突变体。Raf-1突变体的表达阻断了v-Src诱导的转化，这通过恢复为扁平的未转化形态以及在软琼脂中无法形成集落来确定。用缺乏突变Raf-1的亲本载体转染的细胞可被v-Src转化。这些数据表明，v-Src激活的、由Raf-1介导的细胞内信号是v-Src转化所必需的。
