Sano Y, Shimao S
Department of Dermatology, Faculty of Medicine, Tottori University, Yonago, Japan.
J Dermatol Sci. 1993 Feb;5(1):25-31. doi: 10.1016/0923-1811(93)90102-u.
Antigen specific T lymphocytes in delayed type hypersensitivity (DTH) test sites were analyzed in cell-transfer experiments. T lymphocytes from B6 mice were transferred into B6PL mice which differ from B6 at the Thy1 allele. When peritoneal exudate cells (PEC) from ovalbumin (OA)-immunized B6 mice were transferred into B6PL mice intravenously, DTH reaction was observed on footpads. A greater number of B6 T cells was identified at the DTH site. OA-reacting B6 T clones were then transferred with antigens into the footpads of B6PL mice. DTH reaction was induced at the footpads. More T cell clones were identified at the DTH test sites than in the control.
在细胞转移实验中分析了迟发型超敏反应（DTH）测试部位的抗原特异性T淋巴细胞。将来自B6小鼠的T淋巴细胞转移到在Thy1等位基因上与B6不同的B6PL小鼠体内。当将来自卵清蛋白（OA）免疫的B6小鼠的腹腔渗出细胞（PEC）静脉内转移到B6PL小鼠体内时，在足垫上观察到DTH反应。在DTH部位鉴定出更多数量的B6 T细胞。然后将OA反应性B6 T克隆与抗原一起转移到B6PL小鼠的足垫中。在足垫处诱导出DTH反应。在DTH测试部位鉴定出的T细胞克隆比对照组更多。
