Kuse R
Allgemeines Krankenhaus St. Georg, Hämatologische Abteilung, Hamburg, Germany.
Ann Hematol. 1993 Apr;66(4):213-4. doi: 10.1007/BF01703238.
Flow-cytometric reticulocyte counts including their maturation status were performed during follow-up of induction (n = 5) and consolidation (n = 7) polychemotherapy in nine patients with acute myeloid leukemia (AML) using a Sysmex R-3000 automated counter. The reticulocytes fell to an extreme nadir (< 0.01/pl)--as did leukocytes and platelets--and consisted of cells with low fluorescence ratio (LFR) only. After a median interval of 16 days, the fraction with medium fluorescence ratio (MFR) began to rise, preceding the reticulocytes with high fluorescence ratio (HFR) for a median of 1 day in all cases with partial (n = 1) or complete (n = 8) remission. At a median of 7 days after MFR and 5 days after HFR the granulocytes reached the critical limit of > or = 0.5/nl. The reticulocytes rose to the normal range after 9 and 7 days, respectively. Automated flow-cytometric reticulocyte counting including the maturation status has been shown to provide an accessible measure of erythroid activity, which may be of predictive value for granulocyte recovery after aplasiogenic polychemotherapy in AML patients.
在9例急性髓系白血病（AML）患者诱导（n = 5）和巩固（n = 7）多药化疗的随访期间，使用Sysmex R - 3000自动血细胞计数器进行流式细胞术网织红细胞计数，包括其成熟状态。网织红细胞降至极低水平（< 0.01/μl），白细胞和血小板也是如此，并且仅由低荧光比率（LFR）细胞组成。在中位间隔16天后，中等荧光比率（MFR）部分开始上升，在部分缓解（n = 1）或完全缓解（n = 8）的所有病例中，高荧光比率（HFR）网织红细胞比MFR网织红细胞提前中位1天开始上升。在MFR出现后中位7天和HFR出现后中位5天，粒细胞达到≥0.5/μl的临界值。网织红细胞分别在9天和7天后升至正常范围。包括成熟状态的自动流式细胞术网织红细胞计数已被证明可提供一种可获取的红系活性测量方法，这可能对AML患者再生障碍性多药化疗后粒细胞恢复具有预测价值。
