[从接受齐多夫定治疗的患者中分离出的HIV-1逆转录酶基因的突变]

[Mutations of HIV-1 RT gene isolated from patients treated with AZT].

作者信息

Kondo M, Saito T, Ito A, Oka S, Takata N, Imai M

机构信息

Department of Virology, Kanagawa Prefectural Public Health Laboratories.

出版信息

Kansenshogaku Zasshi. 1993 Mar;67(3):185-9. doi: 10.11150/kansenshogakuzasshi1970.67.185.

DOI:10.11150/kansenshogakuzasshi1970.67.185

PMID:7683706

Abstract

HIV-1 strains were isolated from three patients treated with AZT and from three patients not treated with AZT. Progenomes of HIV-1 RT gene were amplified by PCR and cloned to M13mp18 vecter. Four amino acid mutations in RT gene (Asp67, Lys70, Thr215, Lys219) associated with resistance to AZT were analysed. All of the 14 clones obtained from the three patients with AZT therapy had mutations at codon 215 (Thr-->Tyr or Phe). Some of the 14 clones also had other mutations at codon 67 (Asp-->Asn or Ser), codon 70 (Lys-->Arg) and codon 219 (Lys-->Glu). All of 18 clones obtained from the patients not treated with AZT have no mutation at any codon mentioned above.

摘要

HIV-1毒株分别从三名接受齐多夫定（AZT）治疗的患者和三名未接受AZT治疗的患者中分离得到。通过聚合酶链反应（PCR）扩增HIV-1逆转录酶（RT）基因的前基因组，并克隆到M13mp18载体中。分析了RT基因中与对AZT耐药相关的四个氨基酸突变（Asp67、Lys70、Thr215、Lys219）。从三名接受AZT治疗的患者中获得的14个克隆在密码子215处均发生了突变（Thr→Tyr或Phe）。这14个克隆中的一些在密码子67（Asp→Asn或Ser）、密码子70（Lys→Arg）和密码子219（Lys→Glu）处也有其他突变。从未接受AZT治疗的患者中获得的18个克隆在上述任何密码子处均无突变。