Bukauskas F F, Weingart R
Department of Physiology, University of Berne, Switzerland.
Pflugers Arch. 1993 Apr;423(1-2):152-4. doi: 10.1007/BF00374973.
Two cells of an insect cell line (Aedes albopictus, clone C6/36) were pushed together to form a cell pair while the intercellular current flow was monitored. This approach enabled us to study the formation of gap junction channels and explore their electrical properties. We found that the single channels exhibit multiple conductance states. The conductance of a fully open channel was 365 pS; the subconductance steps were 1/7 to 1/5 of the maximal conductance. The voltage gradient across the junction did not influence the conductance of fully open channels, but affected the dwell time at particular conductance states. The latter provides an explanation for the voltage-dependent conductance of gap junction membranes seen in these cells. The very first channel opening always was slow (15-50 ms), suggesting the involvement of a mechanism different from conventional channel gating.
将昆虫细胞系（白纹伊蚊，克隆C6/36）的两个细胞推到一起形成细胞对，同时监测细胞间电流流动。这种方法使我们能够研究间隙连接通道的形成并探索其电学性质。我们发现单通道呈现多种电导状态。完全开放通道的电导为365 pS；亚电导台阶为最大电导的1/7至1/5。跨连接的电压梯度不影响完全开放通道的电导，但影响在特定电导状态下的停留时间。后者为这些细胞中观察到的间隙连接膜的电压依赖性电导提供了解释。最初的通道开放总是缓慢的（15 - 50毫秒），这表明涉及一种不同于传统通道门控的机制。
