Cannon S C, McClatchey A I, Gusella J F
Howard Hughes Medical Institute, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston 02114.
Pflugers Arch. 1993 Apr;423(1-2):155-7. doi: 10.1007/BF00374974.
Sodium channels are multimeric structures composed of alpha and beta subunits. Oocytes injected with RNA encoding only the alpha subunit express voltage-gated Na+ currents. The kinetics of inactivation, however, are abnormal. Co-injection of rat brain alpha and beta subunits modifies inactivation of INa such that it closely resembles endogenous currents. Here we show that a beta subunit derived from human brain directs the same functional modification of INa expressed by a rat skeletal muscle alpha subunit. This implies that functional domains for the interaction of alpha and beta subunits are highly conserved across both tissues and species.
钠通道是由α和β亚基组成的多聚体结构。仅注射编码α亚基的RNA的卵母细胞表达电压门控性Na⁺电流。然而，失活的动力学是异常的。共注射大鼠脑α和β亚基可改变Na⁺电流的失活，使其与内源性电流非常相似。在这里我们表明，源自人脑的β亚基可对由大鼠骨骼肌α亚基表达的Na⁺电流进行相同的功能修饰。这意味着α和β亚基相互作用的功能域在组织和物种之间高度保守。
