Eaker E Y, Sallustio J E, Harris J M, Shaw G
Department of Medicine, College of Medicine, University of Florida, Gainesville 32610-0214.
Neuroscience. 1993 Mar;53(2):561-70. doi: 10.1016/0306-4522(93)90221-z.
We have previously shown that neurofilaments in enteric neurons are immunologically distinct from those found in the central nervous system. In particular, one monoclonal antibody to the medium molecular weight neurofilament subunit, called NN18, stained the perikarya of enteric neurons very weakly, if at all, although other medium molecular weight neurofilament subunit antibodies clearly showed the presence of significant amounts of medium molecular weight neurofilament subunit. We have since located the epitope for NN18 in a peptide sequence at the carboxy terminal tail of medium molecular weight neurofilament subunit and have now looked for further neurofilament antibodies that stain the same region. We found two monoclonal antibodies, RMO1 and RMO59, that recognize the same peptide. These antibodies also show much reduced staining in myenteric neurons compared to those in the central nervous system, suggesting that this region of the medium molecular weight neurofilament subunit is specifically modified in certain cells. In the developing enteric nervous system, we found that all the antibodies show strong staining of enteric neurons in the neonatal rat, but that with NN18, RMO1, and RMO59, the staining intensity decreases during further development, and by postnatal day 21 all three demonstrate decreased or absent staining identical to the adult. These results indicate that myenteric neurons, in contrast to the brain, have a developmentally regulated modification in a specific region of the medium molecular weight neurofilament subunit, which may reflect adaptation to structural stress by myenteric neurons.
我们先前已表明，肠神经元中的神经丝在免疫学上与中枢神经系统中的神经丝不同。特别是，一种针对中分子量神经丝亚基的单克隆抗体，名为NN18，对肠神经元的胞体染色非常弱，即便有染色也极其微弱，尽管其他中分子量神经丝亚基抗体清楚地显示存在大量的中分子量神经丝亚基。此后，我们已将NN18的表位定位在中分子量神经丝亚基羧基末端尾部的一个肽序列中，并且现在寻找了能对同一区域进行染色的其他神经丝抗体。我们发现了两种单克隆抗体，RMO1和RMO59，它们识别相同的肽。与中枢神经系统中的神经元相比，这些抗体在肌间神经丛神经元中的染色也显著减少，这表明中分子量神经丝亚基的这个区域在某些细胞中被特异性修饰。在发育中的肠神经系统中，我们发现所有抗体在新生大鼠的肠神经元中均显示强染色，但对于NN18、RMO1和RMO59，染色强度在进一步发育过程中降低，到出生后第21天，这三种抗体均显示与成年时相同的染色减少或无染色。这些结果表明，与大脑不同，肌间神经丛神经元在中分子量神经丝亚基的特定区域具有发育调控的修饰，这可能反映了肌间神经丛神经元对结构应力的适应性。
