Horton R M, Manfredi A A, Conti-Tronconi B M
Department of Biochemistry, University of Minnesota, St. Paul 55108.
Neurology. 1993 May;43(5):983-6. doi: 10.1212/wnl.43.5.983.
We have PCR-amplified cDNA sequence of the "embryonic" gamma subunit of muscle acetylcholine receptor (AChR) from adult bovine extraocular muscle (EOM). We cloned and sequenced this product and used it to probe Northern blots. We detected the gamma subunit in EOM mRNA, but not in control skeletal muscle. The presence of gamma subunit in EOMs may explain their preferential involvement in myasthenia gravis, where an autoimmune response to the gamma subunit is generally present.
我们从成年牛眼外肌（EOM）中通过聚合酶链反应（PCR）扩增出肌肉乙酰胆碱受体（AChR）“胚胎型”γ亚基的互补脱氧核糖核酸（cDNA）序列。我们对该产物进行了克隆和测序，并用于探测Northern印迹。我们在EOM的信使核糖核酸（mRNA）中检测到了γ亚基，但在对照骨骼肌中未检测到。EOM中γ亚基的存在可能解释了它们在重症肌无力中优先受累，在重症肌无力中通常存在针对γ亚基的自身免疫反应。
