McDonald A R, Garbary D J, Duckett J G
Department of Biology, St. Francis Xavier University, Antigonish, Nova Scotia, Canada.
Biotech Histochem. 1993 Mar;68(2):91-8. doi: 10.3109/10520299309104673.
Rhodamine-phalloidin was used to label F-actin in unfixed cells of 13 species of filamentous and blade-forming red algae from the three families Ceramiaceae, Acrochaetiaceae and Bangiaceae. Labelling was achieved only after treatment with either beta-glucuronidase or a combination of cellulase and an extract of snail gut enzyme. Different species required different enzyme treatments and incubation times for successful labelling. All species examined showed extensive arrays of F-actin which generally are confined to the peripheral cytoplasm and are oriented longitudinally. Transverse arrays are present beside the crosswalls of Griffithsia pacifica, and Audouinella species show actin concentrations at the tips of apical cells and in developing branch initials.
用罗丹明-鬼笔环肽对来自仙菜科、顶丝藻科和红毛菜科的13种丝状和叶片状红藻的未固定细胞中的F-肌动蛋白进行标记。只有在用β-葡萄糖醛酸酶或纤维素酶与蜗牛肠道酶提取物的组合处理后才能实现标记。不同的物种需要不同的酶处理和孵育时间才能成功标记。所有检测的物种都显示出广泛的F-肌动蛋白阵列，这些阵列通常局限于外周细胞质并纵向排列。在太平洋格氏藻的横壁旁边存在横向阵列，顶丝藻属物种在顶端细胞的尖端和发育中的分支起始部位显示出肌动蛋白浓度。
