Baron P, Kamholz J, Scherer S, Honda H, Shy M, Scarpini E, Scarlato G, Pleasure D
Department of Neurology, University of Pennsylvania, Philadelphia 19104.
Exp Neurol. 1993 May;121(1):139-47. doi: 10.1006/exnr.1993.1080.
The capacity of specific regions and fiber tracts of developing rat lumbosacral spinal cord to express proteolipid protein (PLP) mRNA was examined using in situ hybridization. PLP mRNA was undetectable at Day 18 postfertilization but present in the white matter by Postnatal Day 0 (P0). Initially, levels of PLP mRNA were higher in ventral than in dorsal funiculi. Hybridization signal was observed in the corticospinal tract by P10. Appearance of PLP message in gray matter lagged several days behind that in white matter. The expression of PLP mRNA in gray matter rose between P10 and P40 and the abundance of PLP transcript remained high in white matter during the same period. In the adult spinal cord some oligodendrocytes in the Lissauer tract and in the posterior commissure expressed low levels of PLP mRNA, which was undetectable in the substantia gelatinosa. Comparing the time of emergence of PLP mRNA to the histological appearance of myelination gave evidence that the message is expressed in oligodendrocytes that are not many days away from myelin formation. These results provide new information on the topographic distribution of PLP mRNA during myelination of the rat spinal cord.
运用原位杂交技术检测了发育中大鼠腰骶脊髓特定区域和纤维束表达蛋白脂蛋白（PLP）mRNA的能力。受精后第18天未检测到PLP mRNA，但在出生后第0天（P0）白质中已存在。最初，腹侧索中PLP mRNA水平高于背侧索。到出生后第10天（P10），在皮质脊髓束中观察到杂交信号。灰质中PLP信息的出现比白质滞后几天。灰质中PLP mRNA的表达在P10至P40之间上升，同一时期白质中PLP转录本的丰度保持较高。在成年脊髓中，Lissauer束和后连合中的一些少突胶质细胞表达低水平的PLP mRNA，而在胶状质中未检测到。将PLP mRNA出现的时间与髓鞘形成的组织学外观进行比较，结果表明该信息在距离髓鞘形成不远的少突胶质细胞中表达。这些结果为大鼠脊髓髓鞘形成过程中PLP mRNA的拓扑分布提供了新信息。
