Vik H, Steinvåg S K, Elsayed S
Allergy Research Group, University Hospital, University of Bergen, Norway.
Int Arch Allergy Immunol. 1993;101(1):89-94. doi: 10.1159/000236503.
An N-terminal peptide of the major allergen of birch (Bet v I 23-38) was selected for studying the activity of this segment on the basis of optimal hydrophilicity as it was tentatively suggested to be a surface exposed epitope. In addition two control peptides in the region 1-38 were similarly used for comparative assignment of the allergenicity. Peptide analogues from the amino acid terminal region, amino acid residues No. 23-38 of Bet v I, were synthesized by semiautomatic solid-phase peptide synthesis. In vitro and in vivo biological activity studies were performed on these analogous peptides. The IgE-binding capacity of the synthetic peptide 23-38 was examined using the following tests: specific IgE inhibition, skin prick test, nasal provocation and Prausnitz-Küstner inhibition. The results of these investigations suggested that the region 23-38 from the birch and hazel major allergen encompassed a single haptenic epitope.
基于最佳亲水性，选择桦树主要变应原（Bet v I 23 - 38）的N端肽段来研究该片段的活性，因为初步认为它是一个表面暴露表位。此外，1 - 38区域的两个对照肽也同样用于变应原性的比较分析。通过半自动固相肽合成法合成了来自Bet v I氨基酸末端区域（氨基酸残基23 - 38）的肽类似物。对这些类似肽进行了体外和体内生物活性研究。使用以下试验检测合成肽23 - 38的IgE结合能力：特异性IgE抑制试验、皮肤点刺试验、鼻激发试验和普劳斯尼茨 - 屈斯特纳抑制试验。这些研究结果表明，桦树和榛树主要变应原的23 - 38区域包含一个单一的半抗原表位。
