Miadonna A, Roncarolo M G, Lorini M, Tedeschi A
Department of Internal Medicine, Infectious Diseases, and Immunopathology, University of Milan, Ospedale Maggiore Policlinico, Italy.
Clin Immunol Immunopathol. 1993 Jun;67(3 Pt 1):210-5. doi: 10.1006/clin.1993.1067.
The effects of interleukin (IL)-2, -3, -4, -5, -6, and -7 and granulocyte-macrophage colony-stimulating factor (GM-CSF) on histamine release from human basophils were evaluated. IL-3 was the only cytokine with histamine-releasing activity. This activity was observed predominantly on basophils from allergic patients (mean release +/- SEM, 33.9 +/- 9.5%; n = 12), whereas basophils from normal subjects responded less frequently to stimulation with IL-3 (mean release +/- SEM, 2.8 +/- 1.0%; n = 22). The effect of IL-3 was time and temperature dependent, since release was optimal after incubation for 120 min at 37 degrees C. When cell-bound IgE were eluted at acid pH, basophils became unresponsive to IL-3; however, IL-3-induced histamine release correlated with anti-IgE-induced histamine release in allergics, but not in normals. IL-3, IL-5, IL-6, and GM-CSF enhanced significantly anti-IgE- and FMLP-induced histamine release. In contrast, IL-2, IL-4, and IL-7 were devoid of any significant histamine-releasing or -potentiating activity. These results indicate that IL-3 can induce and IL-3, -5, and -6 and GM-CSF can enhance histamine release from human basophils, suggesting a possible role of these cytokines in the expression of allergic reactions.
评估了白细胞介素（IL）-2、-3、-4、-5、-6、-7和粒细胞-巨噬细胞集落刺激因子（GM-CSF）对人嗜碱性粒细胞组胺释放的影响。IL-3是唯一具有组胺释放活性的细胞因子。这种活性主要在过敏患者的嗜碱性粒细胞上观察到（平均释放量±标准误，33.9±9.5%；n = 12），而正常受试者的嗜碱性粒细胞对IL-3刺激的反应频率较低（平均释放量±标准误，2.8±1.0%；n = 22）。IL-3的作用具有时间和温度依赖性，因为在37℃孵育120分钟后释放最为明显。当在酸性pH下洗脱细胞结合的IgE时，嗜碱性粒细胞对IL-3不再有反应；然而，在过敏患者中，IL-3诱导的组胺释放与抗IgE诱导的组胺释放相关，但在正常受试者中不相关。IL-3、IL-5、IL-6和GM-CSF显著增强了抗IgE和FMLP诱导的组胺释放。相比之下，IL-2、IL-4和IL-7没有任何显著的组胺释放或增强活性。这些结果表明，IL-3可以诱导，IL-3、-5、-6和GM-CSF可以增强人嗜碱性粒细胞的组胺释放，提示这些细胞因子在过敏反应表达中可能起作用。
