Obradovich J E, Ogilvie G K, Stadler-Morris S, Schmidt B R, Cooper M F, Boone T C
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
J Vet Intern Med. 1993 Mar-Apr;7(2):65-7. doi: 10.1111/j.1939-1676.1993.tb03171.x.
Recombinant canine granulocyte colony-stimulating factor (rcG-CSF) was administered subcutaneously at a dosage of 5 micrograms/kg/day to five healthy, young adult cats for 42 days. Mean neutrophil counts +/- standard deviation increased significantly (P < 0.001) from 10,966/microL +/- 2324 to 30,688/microL +/- 5296 within 24 hours after administration of the first dosage of rcG-CSF. Mean neutrophil counts reached 52,978/microL +/- 11,207 on day 6, representing a second significant increase (P < 0.01) over the previous 5 days. Mean neutrophil counts continued to increase, reaching 66,994/microL +/- 12,419 on day 14, then remaining within a range of 66,994 to 87,839/microL throughout the remainder of the study. The maximum mean neutrophil count was 87,839/microL +/- 8,695 on day 42. Neutrophil counts remained high until the administration of recombinant canine granulocyte colony-stimulating factor was discontinued 42 days after initiation of therapy. Once the rcG-CSF administration was discontinued, neutrophil counts returned to pretreatment values within 5 days. There were no significant changes in numbers of any of the other cell lines. There was no clinically significant toxicosis associated with the administration of rcG-CSF.
将重组犬粒细胞集落刺激因子（rcG-CSF）以5微克/千克/天的剂量皮下注射给5只健康的年轻成年猫，持续42天。首次注射rcG-CSF后24小时内，平均中性粒细胞计数±标准差从10,966/微升±2324显著增加（P < 0.001）至30,688/微升±5296。第6天时平均中性粒细胞计数达到52,978/微升±11,207，相较于前5天出现第二次显著增加（P < 0.01）。平均中性粒细胞计数持续增加，在第14天时达到66,994/微升±12,419，然后在研究的剩余时间内维持在66,994至87,839/微升的范围内。第42天时最大平均中性粒细胞计数为87,839/微升±8,695。在治疗开始后42天停止注射重组犬粒细胞集落刺激因子之前，中性粒细胞计数一直保持高位。一旦停止注射rcG-CSF，中性粒细胞计数在5天内恢复到治疗前水平。其他任何细胞系的数量均无显著变化。注射rcG-CSF未出现具有临床意义的中毒反应。
