Benichou S, Venet A, Beyer C, Tiollais P, Madaule P
Unité de Recombinaison et Expression Génétique, INSERM U163, Institut Pasteur, Paris, France.
Virology. 1993 Jun;194(2):870-4. doi: 10.1006/viro.1993.1333.
We identified previously a neutralizing epitope in the V2 domain of the simian immunodeficiency virus (SIVmac) external envelope protein. The present study reports identification of five additional linear epitopes of SIVmac (isolate 251) by immunological screening of a peptide library expressed in yeast, using SIVmac-infected macaque sera. Three epitopes were localized in the envelope glycoproteins and the two others in the reverse transcriptase and in the Rev regulatory protein. Antibody response against the four envelope epitopes was monitored for 2 years in 12 macaques experimentally infected by SIVmac251. These four envelope regions represent major immunodominant epitopes of the SIVmac. Two epitopes are located in the V3 domain (a.a. 311-330) of the external gp130 and near the amino terminal part (a.a. 601-619) of the transmembrane gp36, in regions similar to those identified in HIVs, demonstrating immunological similarities between the envelopes of SIVs and HIVs. SIV-specific immunodominant epitopes were also identified in the V1 (a.a. 111-130) and V2 (a.a. 171-190) domains of the external gp130. In particular, antibody response against the V2 neutralizing region seems to play some role in the control of disease progression in SIVmac-infected macaques.
我们先前在猴免疫缺陷病毒（SIVmac）外膜蛋白的V2结构域中鉴定出一个中和表位。本研究报告了通过用感染SIVmac的猕猴血清对酵母中表达的肽库进行免疫筛选，又鉴定出SIVmac（分离株251）的另外五个线性表位。三个表位位于包膜糖蛋白中，另外两个位于逆转录酶和Rev调节蛋白中。在12只经SIVmac251实验感染的猕猴中监测了针对四个包膜表位的抗体反应，为期2年。这四个包膜区域代表了SIVmac的主要免疫显性表位。两个表位位于外膜gp130的V3结构域（氨基酸311 - 330）以及跨膜gp36的氨基末端部分附近（氨基酸601 - 619），这些区域与在HIV中鉴定出的区域相似，表明SIV和HIV包膜之间存在免疫学相似性。在gp130外膜的V1（氨基酸111 - 130）和V2（氨基酸171 - 190）结构域中也鉴定出了SIV特异性免疫显性表位。特别是，针对V2中和区域的抗体反应似乎在控制SIVmac感染猕猴的疾病进展中发挥了一定作用。
