Hinoda Y, Arimura Y, Itoh F, Adachi M, Tsujisaki M, Imai K, Yachi A
Department of Internal Medicine, Sapporo Medical College, Japan.
J Clin Lab Anal. 1993;7(2):100-4. doi: 10.1002/jcla.1860070206.
A monoclonal antibody (MAb) MUSE11 recognizes an epitope in the tandem repeat domain of a mucin core protein, MUC1. We show that the epitope of MAb MUSE11 could be within the continuous amino acid sequence PDTRPAPG. Since there is increasing evidence indicating that this region is highly immunogenic, cDNA cloning of the variable regions of heavy-chain (VH) and of light-chain (VL) of MAb MUSE11 was performed by using RT-PCR to provide a basis for analyzing the structure of the antibody-antigen complex and for producing anti-idiotypic antibodies. The deduced amino acid sequence revealed that the VH and VK of MAb MUSE11 could be assigned to subgroups IIIA and II of mouse immunoglobulin heavy and light chains, respectively. When compared with the V regions of other MAbs in the same subgroup, the complementary determining region 3 (CDR3) in the VH region of MAb MUSE11 consisted of a unique sequence that may be important in defining the specificity of MAb MUSE11.
单克隆抗体（MAb）MUSE11识别粘蛋白核心蛋白MUC1串联重复结构域中的一个表位。我们发现MAb MUSE11的表位可能位于连续氨基酸序列PDTRPAPG内。由于越来越多的证据表明该区域具有高度免疫原性，因此通过逆转录聚合酶链反应（RT-PCR）对MAb MUSE11重链（VH）和轻链（VL）可变区进行cDNA克隆，为分析抗体-抗原复合物的结构及产生抗独特型抗体提供依据。推导的氨基酸序列显示，MAb MUSE11的VH和VK可分别归属于小鼠免疫球蛋白重链和轻链的IIIA亚组和II亚组。与同一亚组中其他单克隆抗体的V区相比，MAb MUSE11 VH区的互补决定区3（CDR3）由独特序列组成，这可能对确定MAb MUSE11的特异性很重要。
