Krushel L A, Johnston J G, Fishell G, Tibshirani R, van der Kooy D
Department of Anatomy, University of Toronto, Ontario, Canada.
Neuroscience. 1993 Apr;53(4):1035-47. doi: 10.1016/0306-4522(93)90487-z.
The role of cell lineage in the organization of the cerebral cortex and striatum of the developing rat forebrain was analysed using retroviral-mediated gene transfer to mark the progeny of individual progenitors. Injections around the onset of neurogenesis (embryonic day 14) produced neuronal- and glial-specific clones in the striatum and cortex. The majority of the neuronal clones were restricted to either the deep or superficial layers of the cortex and to either the striatal patch or matrix compartments of the striatum. Moreover, modeling the distributions of the neuronal clones in various ways revealed that grouping the clones into deep vs superficial cortical compartments and patch vs matrix striatal compartments best accounted for the clone distributions. These results suggest that at the onset of neurogenesis there is a heterogeneity of neuronal progenitors within the proliferative ventricular zone.
利用逆转录病毒介导的基因转移来标记单个祖细胞的后代，分析了细胞谱系在发育中的大鼠前脑大脑皮层和纹状体组织中的作用。在神经发生开始时（胚胎第14天）进行注射，在纹状体和皮层中产生了神经元特异性和胶质细胞特异性克隆。大多数神经元克隆局限于皮层的深层或浅层以及纹状体的纹状小体或基质区室。此外，以各种方式对神经元克隆的分布进行建模表明，将克隆分为皮层深层与浅层区室以及纹状体纹状小体与基质区室最能解释克隆的分布情况。这些结果表明，在神经发生开始时，增殖性脑室区的神经元祖细胞存在异质性。
