大肠杆菌中“RNA 控制”位点对 RNA 噬菌体 R23 复制的影响。
Effect of the "RNA control" locus in Escherichia coli on RNA bacteriophage R23 replication.
作者信息
Ernberg J, Sköld O
出版信息
J Virol. 1976 Feb;17(2):307-15. doi: 10.1128/JVI.17.2.307-315.1976.
Abstract
The effect of the rel gene of Escherichia coli on the RNA synthesis induced by phage R23 was studied. This RNA phage has the property of inhibiting ribosomal RNA formation and completely dominating the RNA synthesis of the host. Phage-specific RNA formation was found to be dependent on the allelic state of the rel gene. Determinations of RNA synthesis were made by both cumulative and short-term incorporations of uracil and adenine. Variations in labeling of nucleotide pools were compensated for by determining specific activities of ATP and UTP and using these values to obtain true, relative rates of RNA synthesis.
摘要
研究了大肠杆菌rel基因对噬菌体R23诱导的RNA合成的影响。这种RNA噬菌体具有抑制核糖体RNA形成并完全主导宿主RNA合成的特性。发现噬菌体特异性RNA的形成依赖于rel基因的等位基因状态。通过尿嘧啶和腺嘌呤的累积掺入和短期掺入来测定RNA合成。通过测定ATP和UTP的比活性并使用这些值来获得RNA合成的真实相对速率，补偿了核苷酸池标记的变化。
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