Kindmark A, Törmä H, Johansson A, Ljunghall S, Melhus H
Department of Internal Medicine, University Hospital, Uppsala, Sweden.
Biochem Biophys Res Commun. 1993 May 14;192(3):1367-72. doi: 10.1006/bbrc.1993.1567.
The 9-cis retinoic acid receptor (RXR) alpha, a co-regulator of the thyroid hormone and vitamin D receptors, has previously been shown to be expressed predominantly in metabolic organs such as the liver and the kidney. In this study we have used a reverse transcription polymerase chain reaction (RT-PCR) to examine the expression of retinoic acid (RA) nuclear receptors in primary human osteoblasts and in SaOS-2, a human osteosarcoma-derived cell line with osteoblastic characteristics. Our results demonstrate that human osteoblasts express RXR alpha, as well as the all-trans RA receptors (RAR) alpha, beta, and gamma. These data further establish bone as a major target for retinoids and suggest that RA can regulate vitamin D and thyroid hormone actions in osteoblasts.
9-顺式视黄酸受体（RXR）α是甲状腺激素和维生素D受体的一种共同调节因子，此前已表明其主要在肝脏和肾脏等代谢器官中表达。在本研究中，我们使用逆转录聚合酶链反应（RT-PCR）来检测视黄酸（RA）核受体在原代人成骨细胞以及SaOS-2（一种具有成骨细胞特征的人骨肉瘤衍生细胞系）中的表达。我们的结果表明，人成骨细胞表达RXRα以及全反式RA受体（RAR）α、β和γ。这些数据进一步确定骨骼是类视黄醇的主要靶器官，并表明RA可调节成骨细胞中维生素D和甲状腺激素的作用。
