Madrigal-Bujaidar E, Cadena S R, Trujillo-Valdés V M, Cassani M
Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, I.P.N. Carpio y Plan de Ayala, México, D.F.
Mutat Res. 1993 Jul;300(2):135-40. doi: 10.1016/0165-1218(93)90131-v.
Metrifonate is a drug widely used to cure several helminthiases in man including cysticercosis. In this study we determine its capacity to induce SCEs in vivo and in vitro using the fluorescence-plus-Giemsa technique. In the human lymphocyte system we tested 10, 20, 30, 40, 50 and 60 micrograms/ml added at 24 h of culture with a duration of 72 h. The results showed a SCE inducing effect with the exception of the first tested dose (10 micrograms/ml), but without a dose-response expression. A cell cycle progression delay was seen with the three highest dosages. In the mouse bone marrow we tested 30, 60 and 120 mg/kg in a 24-h experiment, and the results indicated an SCE increase only with the highest dose. No modification of the cell proliferation kinetics was observed.
敌百虫是一种广泛用于治疗人类多种蠕虫病（包括囊尾蚴病）的药物。在本研究中，我们使用荧光加吉姆萨技术测定其在体内和体外诱导姐妹染色单体交换（SCEs）的能力。在人类淋巴细胞系统中，我们在培养24小时时添加10、20、30、40、50和60微克/毫升，培养持续72小时。结果显示，除了第一个测试剂量（10微克/毫升）外，均有SCE诱导作用，但无剂量反应表达。最高的三个剂量出现了细胞周期进程延迟。在小鼠骨髓中，我们在24小时的实验中测试了30、60和120毫克/千克，结果表明仅最高剂量使SCE增加。未观察到细胞增殖动力学的改变。
