Li M L, Cardoso A, Sansilvestri P, Hatzfeld A, Kisselev S, Batard P, Levesque J P, Hatzfeld J
Laboratoire CNRS de Biologie Cellulaire et Moléculaire des Facteurs de Croissance, ICIG, Hôpital Paul-Brousse, Villejuif, France.
Nouv Rev Fr Hematol (1978). 1993 Feb;35(1):81-6.
Steel factor (SF), the ligand for the c-kit, also called kit ligand, stem cell factor, or mast cell growth factor, was evaluated on colony formation alone or in combination with other cytokines, from purified human hematopoietic CD34+ cells in low density cell culture. SF alone had a slight effect on granulocyte (G) and macrophage (M) colony formation. It synergized with other cytokines on colony formation from colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte (CFU-GEMM), erythroid and granulocyte-macrophage (CFU-GM) progenitors. However, combination of SF with lineage-specific factors, such as erythropoietin (Epo) or/and granulocyte colony-stimulating factor (G-CSF) was not sufficient for the proliferation of multipotential progenitors (CFU-GEMM). These multipotential progenitors required the presence of multi-lineage factors, such as interleukin 3 (IL3) or granulocytic-macrophage CSF(GM-CSF) for their development.
钢因子（SF）是c-kit的配体，也被称为kit配体、干细胞因子或肥大细胞生长因子，在低密度细胞培养中，对从纯化的人造血CD34+细胞中单独或与其他细胞因子联合形成集落的情况进行了评估。单独的SF对粒细胞（G）和巨噬细胞（M）集落形成有轻微影响。它与其他细胞因子协同作用，促进粒-红-巨噬-巨核集落形成单位（CFU-GEMM）、红系和粒-巨噬集落形成单位（CFU-GM）祖细胞的集落形成。然而，SF与谱系特异性因子，如促红细胞生成素（Epo）或/和粒细胞集落刺激因子（G-CSF）联合，不足以促进多能祖细胞（CFU-GEMM）的增殖。这些多能祖细胞的发育需要多谱系因子的存在，如白细胞介素3（IL3）或粒-巨噬细胞集落刺激因子（GM-CSF）。
