Calaminici M, Abdulla F A, Sinden J D, Stephenson J D
Department of Neuroscience, Institute of Psychiatry, London, UK.
Neuroreport. 1993 May;4(5):585-7. doi: 10.1097/00001756-199305000-00032.
True Blue, a retrograde fluorescent tracer, was used to investigate axonal outgrowth after grafting embryonic forebrain cholinergic tissue into the cholinergically-deafferented rat frontal cortex. Unilateral deafferentation was achieved by injecting alpha-amino-3-OH-4-isoxozole propionic acid (AMPA) into the ipsilateral nucleus basalis magnocellularis. Grafting occurred 3 weeks after the lesion. Eight to 10 weeks later, True Blue was injected bilaterally into the cortex at a superficial site which, on the transplanted side, was located at least 2 mm away from the transplant. Forty-eight h later, retrogradely labelled fluorescent neurones could be seen in the deepest part of the transplants. Some of these neurones were shown to be ChAt-positive, providing evidence of axonal outgrowth from transplanted cholinergic neurones into cholinergically-deafferented frontal cortex.
真蓝，一种逆行荧光示踪剂，被用于研究将胚胎前脑胆碱能组织移植到胆碱能去传入大鼠额叶皮质后轴突的生长情况。通过向同侧大细胞基底核注射α-氨基-3-羟基-4-异恶唑丙酸（AMPA）实现单侧去传入。损伤后3周进行移植。8至10周后，将真蓝双侧注射到皮质浅表部位，在移植侧，该部位距离移植物至少2毫米。48小时后，在移植物最深层可见逆行标记的荧光神经元。其中一些神经元被证明是ChAT阳性，这为移植的胆碱能神经元向胆碱能去传入的额叶皮质长出轴突提供了证据。
