Snoeck H W, Lardon F, Van Bockstaele D R, Peetermans M E
Laboratory of Experimental Hematology, University of Antwerp (UZA), Edegem, Belgium.
Exp Hematol. 1993 May;21(5):635-9.
We compared the myelopoietic effects of interleukin-4 (IL-4) on CD34+HLA-DR+ and on CD34+HLA-DR++ bone marrow progenitor cells stimulated by either granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF) or interleukin-3 (IL-3). IL-4 stimulates G-CSF-induced colony-forming unit-granulocyte (CFU-G) and inhibits all colony types induced by GM-CSF and IL-3 in the HLA-DR++ population, but not in the HLA-DR+ population. In CD34+HLA-DR+ cells, however, a stimulation of G-CSF-supported CFU-G was also seen with sequential application of IL-4 in liquid cultures followed by G-CSF in agar cultures. In order to confirm that these are direct effects of IL-4, single-cell culture experiments were performed with CD34+HLA-DR++ cells. In these cultures IL-4 stimulates G-CSF-induced CFU-G and only inhibits colony-forming unit-macrophage (CFU-M) regardless of the CSF used to generate them.
我们比较了白细胞介素-4（IL-4）对粒细胞集落刺激因子（G-CSF）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）或白细胞介素-3（IL-3）刺激的CD34+HLA-DR+和CD34+HLA-DR++骨髓祖细胞的骨髓生成作用。IL-4刺激G-CSF诱导的粒细胞集落形成单位（CFU-G），并抑制HLA-DR++群体中GM-CSF和IL-3诱导的所有集落类型，但不抑制HLA-DR+群体中的集落类型。然而，在CD34+HLA-DR+细胞中，在液体培养中先应用IL-4，然后在琼脂培养中应用G-CSF，也观察到对G-CSF支持的CFU-G的刺激作用。为了证实这些是IL-4的直接作用，对CD34+HLA-DR++细胞进行了单细胞培养实验。在这些培养物中，IL-4刺激G-CSF诱导的CFU-G，并且仅抑制巨噬细胞集落形成单位（CFU-M），而不管用于生成它们的集落刺激因子如何。
