Seabrook G R, Fong T M
Merck Sharp & Dohme Research Laboratories, Neuroscience Research Centre, Terlings Park, Harlow, Essex, UK.
Neurosci Lett. 1993 Apr 2;152(1-2):9-12. doi: 10.1016/0304-3940(93)90470-6.
Neurokinin receptors can couple to several second messenger systems including phospholipase C and intracellular calcium mobilisation. Using FURA-2 microspectrofluorimetry, this study examines the mobilisation of calcium in CHO cells which had been stably transfected with the long isoform of the human NK1 receptor. Substance P caused a concentration-dependent rise in inositol phosphate production which was correlated with a reversible increase in intracellular calcium levels. The mobilisation of calcium was reduced by the removal of extracellular calcium from the bathing solution, and almost totally abolished by depletion of intracellular calcium pools with 1 microM thapsigargin. These data suggest that the transduction mechanism associated with NK1 (long) receptor activation can utilise both intracellular and extracellular calcium pools in this expression system.
神经激肽受体可与多种第二信使系统偶联，包括磷脂酶C和细胞内钙动员。本研究使用FURA-2显微分光荧光测定法，检测了稳定转染人NK1受体长亚型的CHO细胞中的钙动员情况。P物质引起肌醇磷酸生成呈浓度依赖性升高，这与细胞内钙水平的可逆性增加相关。通过去除浴液中的细胞外钙，钙动员减少，而用1微摩尔毒胡萝卜素耗尽细胞内钙库后，钙动员几乎完全消除。这些数据表明，在该表达系统中，与NK1（长）受体激活相关的转导机制可利用细胞内和细胞外钙库。
