White-Scharf M E, Potts B J, Smith L M, Sokolowski K A, Rusche J R, Silver S
Repligen Corporation, Cambridge, Massachusetts 02139.
Virology. 1993 Jan;192(1):197-206. doi: 10.1006/viro.1993.1022.
The third hypervariable region (V3) of the HIV-1 envelope gp120 protein contains the principal neutralizing domain. Most neutralizing antibodies directed toward this region are very type-specific. Conserved sequences do exist within this region, however, and may prove useful in developing vaccines and therapeutic monoclonal antibodies (MABs) capable of targeting diverse HIV-1 isolates. We have used synthetic peptides containing conserved V3 sequences as immunogens to produce a panel of neutralizing MABs. The characterization of these MABs is described here. In addition, a series of in vitro assays has been developed that may be useful in predicting the neutralization potential of individual antibodies.
HIV-1包膜糖蛋白gp120的第三个高变区（V3）包含主要的中和结构域。大多数针对该区域的中和抗体具有很强的型特异性。不过，该区域内确实存在保守序列，可能在开发能够靶向多种HIV-1分离株的疫苗和治疗性单克隆抗体（MAB）方面发挥作用。我们已将含有V3保守序列的合成肽用作免疫原，以制备一组中和性单克隆抗体。本文描述了这些单克隆抗体的特性。此外，还开发了一系列体外检测方法，可能有助于预测单个抗体的中和潜力。
