Poss M T, Swanson J L, Telen M J, Lasky L C, Vallera D A
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis.
Vox Sang. 1993;64(4):231-9. doi: 10.1111/j.1423-0410.1993.tb03061.x.
A mouse IgG1 monoclonal antibody (MAb) UMRh, was prepared by immunizing Balb/c mice with the Jurkat T cell acute lymphoblastic leukemia (T-ALL) cell line. The MAb UMRh is directed against a widely distributed Rh-related cell surface antigen, present on red blood cells (RBCs) expressing the more common Rh phenotypes. The antigen has reduced expression on RBCs of -D-, DCW-/DCW-, Rhmod and Rhnull phenotypes. UMR immunoblotted a unique pattern on RBC membrane preparations of two bands at 40 and 43 kD and a diffuse pattern extending upward to about 55 kD. The UMRh antigen is also present on peripheral blood mononuclear cells, granulocytes, platelets, leukemic cells of T cell, B cell and myeloid origins, hematopoietic stem cells, and two tumor lines (lung and colon carcinoma). The number of UMRh sites per RBC (CDe/ce) was determined to be 5,519 copies/cell, whereas the sites on a -D- phenotype RBC were 1,096 copies/cell. A T-ALL line (CEM) expressed 333,364 copies/cell and a myeloid line (KG-1) expressed 90,913 copies/cell. Several Rh-related murine MAbs have been described, but our data indicates that UMRh recognizes a previously uncharacterized Rh-related specificity.
通过用Jurkat T细胞急性淋巴细胞白血病（T-ALL）细胞系免疫Balb/c小鼠制备了一种小鼠IgG1单克隆抗体（MAb）UMRh。MAb UMRh针对一种广泛分布的Rh相关细胞表面抗原，该抗原存在于表达更常见Rh表型的红细胞（RBC）上。该抗原在-D-、DCW-/DCW-、Rhmod和Rhnull表型的RBC上表达降低。UMR在RBC膜制剂上免疫印迹出两条带，分别位于40和43 kD，以及一条向上延伸至约55 kD的弥散带。UMRh抗原也存在于外周血单核细胞、粒细胞、血小板、T细胞、B细胞和髓系来源的白血病细胞、造血干细胞以及两种肿瘤细胞系（肺癌和结肠癌细胞系）上。每个RBC（CDe/ce）上UMRh位点的数量被确定为5519个拷贝/细胞，而-D-表型RBC上的位点为1096个拷贝/细胞。一个T-ALL细胞系（CEM）表达333364个拷贝/细胞，一个髓系细胞系（KG-1）表达90913个拷贝/细胞。已经描述了几种Rh相关的鼠单克隆抗体，但我们的数据表明UMRh识别一种以前未被表征的Rh相关特异性。
