Nataf V, Mercier P, Ziller C, Le Douarin N M
Institut d'Embryologie Cellulaire et Moléculaire du CNRS et du Collège de France, Nogent-sur-Marne.
Exp Cell Res. 1993 Jul;207(1):171-82. doi: 10.1006/excr.1993.1177.
In order to produce markers of melanoblasts and melanocytes, we immunized a mouse with trunk neural crest cells differentiated into melanocytes in culture. We obtained three monoclonal antibodies which react both with pigmented and unpigmented cells in epidermis and in neural crest cultures. These markers are characteristic of distinct differentiation steps. One of them, melanoblast/cyte early marker (MelEM) monoclonal antibody (Mab) detects melanoblasts as soon as they have reached the subectodermal mesenchyme, while the two others, Mel1 and Mel2 Mabs, detect antigens present in melanosomes and promelanosomes later in the differentiation process. Moreover, MelEM is a specific marker of melanoblasts/cytes deriving exclusively from neural crest and not of those from the neural retina. The antigen recognized by MelEM Mab is a protein of M(r) 26,000. The epitopes recognized by Mel1 and Mel2 Mabs are carbohydrate moieties carried by glycoproteins of M(r) 123,000 and 85,000, respectively.
为了产生成黑素细胞和黑素细胞的标志物，我们用在培养中分化为黑素细胞的躯干神经嵴细胞免疫小鼠。我们获得了三种单克隆抗体，它们可与表皮和神经嵴培养物中的色素沉着和未色素沉着细胞发生反应。这些标志物是不同分化阶段的特征。其中之一，成黑素细胞/黑素细胞早期标志物（MelEM）单克隆抗体（Mab）一旦成黑素细胞到达外胚层下间充质就可检测到它们，而另外两种，Mel1和Mel2 Mabs，则在分化过程后期检测存在于黑素小体和前黑素小体中的抗原。此外，MelEM是仅源自神经嵴而非源自神经视网膜的成黑素细胞/黑素细胞的特异性标志物。MelEM Mab识别的抗原是一种分子量为26,000的蛋白质。Mel1和Mel2 Mabs识别的表位分别是分子量为123,000和85,000的糖蛋白携带的碳水化合物部分。
