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An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm.

作者信息

Ozawa N, Kano T, Taga C, Hattori M, Sakaki Y, Suzuki H

机构信息

Shionogi Institute for Medical Science, Osaka, Japan.

出版信息

FEBS Lett. 1993 Jul 5;325(3):303-8. doi: 10.1016/0014-5793(93)81094-g.

DOI:10.1016/0014-5793(93)81094-g
PMID:7686513
Abstract

We have developed an exon-trapping system with a newly constructed trapping vector containing multiple cloning sites (designated pEXT2). The system revealed high sensitivity for trapping a control exon from several hundred kbp of DNA. We have applied the system to the cosmid clones located on human chromosome 21p11-q21, and identified two fragments highly homologous to neurofibromatosis 1 (NF1) gene and a clearly transcribed fragment hybridized with approximately 1.6 kb RNA from human brain and human glioblastoma A172 cell.

摘要

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An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm.
FEBS Lett. 1993 Jul 5;325(3):303-8. doi: 10.1016/0014-5793(93)81094-g.
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