普通脱硫弧菌（希登伯勒菌株）中铁氢化酶操纵子：转录本图谱及表达调控

The operon for the Fe-hydrogenase in Desulfovibrio vulgaris (Hildenborough): mapping of the transcript and regulation of expression.

作者信息

van den Berg W A, Stokkermans J P, van Dongen W M

机构信息

Department of Biochemistry, Agricultural University, Wageningen, The Netherlands.

出版信息

FEMS Microbiol Lett. 1993 Jun 1;110(1):85-90. doi: 10.1111/j.1574-6968.1993.tb06299.x.

DOI:10.1111/j.1574-6968.1993.tb06299.x

PMID:7686524

Abstract

The genes for the subunits of the Fe-only hydrogenase from Desulfovibrio vulgaris are transcribed as a 1.9 kb mRNA; the operon contains no other genes besides those encoding the two subunits. The transcriptional start site of the operon was mapped. Determination of hydrogenase activity and hydrogenase mRNA levels indicates a growth-phase dependent regulation of hydrogenase expression at transcriptional level. However, it has not yet been possible to localize the sequences required for regulation and expression of the genes.

摘要

来自普通脱硫弧菌的仅含Fe的氢化酶亚基的基因转录为1.9 kb的mRNA；该操纵子除了编码两个亚基的基因外不包含其他基因。绘制了该操纵子的转录起始位点。氢化酶活性和氢化酶mRNA水平的测定表明，在转录水平上氢化酶表达存在生长阶段依赖性调节。然而，尚未能够定位基因调节和表达所需的序列。