Ahn Y S, Zerban H, Bannasch P
Abteilung für Cytopathologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1993;63(6):351-7. doi: 10.1007/BF02899283.
Sequential changes in the expression of two glucose transporter isoforms (GLUT1, GLUT2), and in the activities of hexokinase, pyruvate kinase and malic enzyme during the development of rat renal basophilic cell tumors were studied using histochemical techniques. Early basophilic cell tubules are similar to proximal convoluted tubules (PCT) in their overall histochemical pattern, particularly in the expression of glucose transporters, suggesting that basophilic cell tubules and tumors derived from them arise from PCT. In comparison with PCT, basophilic cell tubules show slightly increased activities of all the enzymes studied. In basophilic cell tumors, markedly elevated hexokinase and pyruvate kinase activities are accompanied by a considerable reduction in the expression of GLUT2. GLUT1 expression is not found in basophilic cell tubules or PCT. Small basophilic cell tumors also do not express GLUT1, but GLUT1 is regularly expressed in several cell layers surrounding necrotic areas within large basophilic cell tumors. Our results indicate that increased glycolytic activity and reduced GLUT2 expression take place during the development of renal basophilic cell tumors.
采用组织化学技术研究了大鼠肾嗜碱性细胞瘤发生过程中两种葡萄糖转运体异构体（GLUT1、GLUT2）的表达变化，以及己糖激酶、丙酮酸激酶和苹果酸酶的活性变化。早期嗜碱性细胞小管在整体组织化学模式上与近端曲管（PCT）相似，特别是在葡萄糖转运体的表达方面，这表明嗜碱性细胞小管及其衍生的肿瘤起源于PCT。与PCT相比，嗜碱性细胞小管中所研究的所有酶的活性略有增加。在嗜碱性细胞瘤中，己糖激酶和丙酮酸激酶活性显著升高，同时GLUT2的表达大幅降低。在嗜碱性细胞小管或PCT中未发现GLUT1表达。小的嗜碱性细胞瘤也不表达GLUT1，但在大的嗜碱性细胞瘤内坏死区域周围的几层细胞中GLUT1呈规律性表达。我们的结果表明，在肾嗜碱性细胞瘤发生过程中糖酵解活性增加，GLUT2表达降低。
