Dai C
Institute of Medical Biology, Kunming.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1993 Feb;15(1):34-8.
The mouse-adapted Lansing strain of poliovirus type 2 PV-2(L) induces fatal poliomyelitis in mice after intracerebral inoculation, while mice inoculated with Mahoney strain of poliovirus type 1 PV-1(M) show no signs of disease. Previous work had indicated that both the 5' non-translated region of the viral genome and the viral capsid protein, neutralization antigenic site I (N-Ag1), were involved in mouse neurovirulence. In order to further explore the role of N-Ag1 in mouse neurovirulence, antigenic chimeras of two poliovirus strains, XF414 and XF324, were constructed. In the two strains, ten amino acids (in XF414) or 16 amino acids (in XF324) in antigenic site I in Vp1 of PV-I (M) were replaced with a PV-2(L)-specific sequences using a mutagenesis cartridge. Mouse neurovirulence tests indicated that mice cerebral inoculated with XF414 and XF324 developed poliomyelitis leading to paralysis or death. The viruses possessing antigenicity of the inoculating viruses were isolated from cerebral tissues of the paralyzed mice. The results demonstrated that N-Ag1 is an important determinant of poliovirus host range, and may be involved in attachment and penetration of poliovirus (in)to cells of the mouse central nervous system.
2型脊髓灰质炎病毒的小鼠适应株兰辛株（PV - 2(L)）经脑内接种后可在小鼠中诱发致命的脊髓灰质炎，而接种1型脊髓灰质炎病毒马奥尼株（PV - 1(M)）的小鼠则无疾病迹象。先前的研究表明，病毒基因组的5'非翻译区和病毒衣壳蛋白中和抗原位点I（N - Ag1）均与小鼠神经毒力有关。为了进一步探究N - Ag1在小鼠神经毒力中的作用，构建了两种脊髓灰质炎病毒株XF414和XF324的抗原嵌合体。在这两种毒株中，使用诱变盒将PV - 1(M)的Vp1中抗原位点I的10个氨基酸（在XF414中）或16个氨基酸（在XF324中）替换为PV - 2(L)特异性序列。小鼠神经毒力试验表明，经脑内接种XF414和XF324的小鼠发生脊髓灰质炎，导致瘫痪或死亡。从瘫痪小鼠的脑组织中分离出具有接种病毒抗原性的病毒。结果表明，N - Ag1是脊髓灰质炎病毒宿主范围的重要决定因素，可能参与脊髓灰质炎病毒进入小鼠中枢神经系统细胞的附着和穿透过程。
