Moreno A, Clavijo P, Edelman R, Davis J, Sztein M, Sinigaglia F, Nardin E
Department of Medical and Molecular Parasitology, New York University School of Medicine, NY 10010.
J Immunol. 1993 Jul 1;151(1):489-99.
CD4+ T cell clones were derived from three volunteers who were protected against malaria after immunization with Plasmodium falciparum sporozoites. T cells specific for an epitope, Pf Th/Tc, contained in amino acids 326 to 345 of the circumsporozoite (CS) protein of P. falciparum (NF54) were derived from all three volunteers. DR1-, -4-, -7-, and -9-restricted T cell clones were found to recognize overlapping, but distinct, epitopes within a 20-mer peptide representing the amino acid 326 to 345 sequence. The Pf Th/Tc epitope contains part of the highly conserved region II as well as part of a polymorphic domain of the P. falciparum CS protein. All of the overlapping epitopes within peptide 326-345 contained at least three amino acids of the amino terminus of the conserved region II, in addition to a variable number of amino acids in the polymorphic region. The DR4-, -7-, and -9-restricted but not the DR1-restricted T cell clones recognized variant peptides representing this polymorphic region of the CS protein of P. falciparum isolates from Africa, Asia, and South America.
CD4+ T细胞克隆源自三名志愿者，他们在用恶性疟原虫子孢子免疫后对疟疾具有抵抗力。针对恶性疟原虫（NF54）环子孢子（CS）蛋白氨基酸326至345中包含的一个表位Pf Th/Tc的T细胞，源自所有三名志愿者。发现DR1、DR4、DR7和DR9限制性T细胞克隆可识别代表氨基酸326至345序列的20肽内重叠但不同的表位。Pf Th/Tc表位包含高度保守的区域II的一部分以及恶性疟原虫CS蛋白多态性结构域的一部分。除了多态性区域中可变数量的氨基酸外，肽326 - 345内所有重叠表位都包含保守区域II氨基末端的至少三个氨基酸。DR4、DR7和DR9限制性而非DR1限制性T细胞克隆识别代表来自非洲、亚洲和南美洲的恶性疟原虫分离株CS蛋白该多态性区域的变体肽。
