Zhang X B, Ohta Y
Laboratory for Microbial Biochemistry, Faculty of Applied Biological Science, Hiroshima University, Higashi, Japan.
Mutat Res. 1993 Aug;300(3-4):201-6. doi: 10.1016/0165-1218(93)90051-e.
The possible anticlastogenic activity and bio-antimutagenic mechanism of the cultured broth of Saccharomyces cerevisiae 28 were examined using in in vivo and in vitro test systems. In the Ames test with Salmonella typhimurium TA100 (SD-) and in the umu-test with S. typhimurium TA1535/psk1002, the cultured broth of S. cerevisiae 28 showed bio-antimutagenic activity against mutagenicity induced by 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) and 2-amino-3-methylimidazo[4,5-f]quinoline (IQ). The cultured broth also showed bio-antimutagenic activity towards reverse mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Escherichia coli B/r WP2 trp-, but not by UV radiation. It is clear that the cultured broth could inhibit base substitution mutations induced by mutagens. Using mitomycin C (MMC) as a mutagen, the micronucleus test (with bone marrow cells of mice) showed anticlastogenic action when the cultured broth was given orally to mice. Micronucleated polychromatic erythrocytes induced by the mutagen were reduced by about 47% by the cultured broth.
利用体内和体外测试系统，研究了酿酒酵母28培养液可能的抗断裂活性和生物抗诱变机制。在鼠伤寒沙门氏菌TA100（SD-）的艾姆斯试验以及鼠伤寒沙门氏菌TA1535/psk1002的umu试验中，酿酒酵母28的培养液对由3-氨基-1,4-二甲基-5H-吡啶并[4,3-b]吲哚（Trp-P-1）、3-氨基-1-甲基-5H-吡啶并[4,3-b]吲哚（Trp-P-2）和2-氨基-3-甲基咪唑并[4,5-f]喹啉（IQ）诱导的诱变性表现出生物抗诱变活性。该培养液对大肠杆菌B/r WP2 trp-中由N-甲基-N'-硝基-N-亚硝基胍（MNNG）诱导的回复突变也表现出生物抗诱变活性，但对紫外线辐射诱导的回复突变则没有。显然，该培养液能够抑制诱变剂诱导的碱基置换突变。以丝裂霉素C（MMC）作为诱变剂，微核试验（用小鼠骨髓细胞）表明，当给小鼠口服该培养液时，其具有抗断裂作用。诱变剂诱导的微核多色红细胞数量被该培养液减少了约47%。
