Cairo M S, Gillan E R, Buzby J S, van de Ven C, Suen Y
Division of Hematology/Oncology, Children's Hospital of Orange County, CA 92668.
Am J Pediatr Hematol Oncol. 1993 Aug;15(3):311-5.
An immaturity in humoral, cellular, and phagocytic immunity predisposes the newborn to overwhelming bacterial infection. The maturation and proliferation of early hemapoietic stem cells give rise to all three of these aspects of immunity. Defects in the regulation of early hematopoiesis may account in part for the immaturity of neonatal host defense. A new hematopoietic growth factor, Steel factor (SLF), has recently been demonstrated to induce the proliferation of early hematopoietic progenitor cells. Our purpose was to study SLF and its effect on hematopoiesis.
We measured circulating serum SLF levels in preterm and term newborns and compared them to adults, matched third trimester pregnant mothers, and to circulating granulocyte colony-stimulating factor (G-CSF) levels.
There was no significant difference in SLF levels between preterm and term newborns and adults (3000 +/- 200 vs. 2700 +/- 200 vs. 3100 +/- 300 pg/ml) (Pt vs. T vs. A) (p = NS). Also, there was no significant difference between matched third trimester maternal levels and their matched term newborn (2650 +/- 330 pg/ml vs. 3530 +/- 400 pg/ml) (p = NS). However, G-CSF levels were significantly higher in preterm newborns compared to term newborns and adults (p < or = 0.025). The preterm newborn G-CSF levels, however, were significantly lower compared to positive controls obtained from neutropenic patients post bone marrow transplantation (ANC < or = 200/mm3) (174 +/- 86 vs. 669 +/- 82.3 pg/ml) (p < 0.001). Additionally, there were no significant differences in G-CSF levels between matched third trimester maternal samples and matched term newborns.
These studies suggest that circulating levels of SLF in the preterm and term newborn are similar to adults and do not account for differences in hematopoiesis.
体液免疫、细胞免疫和吞噬免疫的不成熟使新生儿易患严重细菌感染。早期造血干细胞的成熟和增殖产生了免疫的这三个方面。早期造血调节缺陷可能部分解释了新生儿宿主防御的不成熟。一种新的造血生长因子，即 Steel 因子（SLF），最近已被证明可诱导早期造血祖细胞增殖。我们的目的是研究 SLF 及其对造血的影响。
我们测量了早产儿和足月儿的循环血清 SLF 水平，并将其与成年人、孕晚期匹配的母亲以及循环粒细胞集落刺激因子（G-CSF）水平进行比较。
早产儿、足月儿和成年人的 SLF 水平无显著差异（3000±200 与 2700±200 与 3100±300 pg/ml）（早产 vs. 足月 vs. 成人）（p = 无显著性差异）。此外，孕晚期母亲与其匹配的足月儿的 SLF 水平也无显著差异（2650±330 pg/ml 与 3530±400 pg/ml）（p = 无显著性差异）。然而，早产儿的 G-CSF 水平显著高于足月儿和成年人（p≤0.025）。不过，与骨髓移植后中性粒细胞减少患者（绝对中性粒细胞计数≤200/mm³）的阳性对照相比，早产儿的 G-CSF 水平显著较低（174±86 与 669±82.3 pg/ml）（p＜0.001）。此外，孕晚期母亲样本与其匹配的足月儿的 G-CSF 水平无显著差异。
这些研究表明，早产儿和足月儿的循环 SLF 水平与成年人相似，且不能解释造血方面的差异。
