Diaz-Ricart M, Tandon N N, Carretero M, Ordinas A, Bastida E, Jamieson G A
Hospital Clinic I Provincial, Facultad de Medicina, Universidad de Barcelona, Spain.
Blood. 1993 Jul 15;82(2):491-6.
A parallel-plate perfusion chamber has been used to evaluate the contribution of the adhesive membrane glycoprotein CD36 (GPIV) to platelet adhesion on type I collagen in flowing whole blood at a shear rate of 800 s-1. In one series of experiments, reconstituted normal blood (hematocrit 0.4; platelet count 1.5 x 10(5)/microL) was prepared from washed red blood cells, plasma, and washed platelets that had been incubated with Fab fragments of a monospecific polyclonal anti-CD36 antibody (50 micrograms/mL, 30 minutes, 37 degrees C). Percent surface coverage of collagen-coated coverslips using reconstituted blood with antibody-blocked platelets, as compared with paired reconstituted controls (100%), was 50% at 2 minutes, 87% at 5 minutes, and 90% at 10 minutes. Further studies were performed by perfusion of whole blood from a healthy donor of the Naka-negative phenotype, whose platelets constitutively lack CD36, over collagen-coated coverslips. In this case, percent surface coverage was 55% of normal controls at 2 minutes, 76% of controls at 5 minutes, and 72% of controls at 10 minutes. In both preparations, platelets lacking functional CD36 had a statistically significant decrease (P < .005) in adhesion after 2 minutes and 10 minutes perfusion but not at 5 minutes. These results show that functional CD36 facilitates the rapid adhesion of platelets to collagen and that this effect is seen at the earliest time points of their interaction.
一种平行板灌注腔已被用于评估黏附膜糖蛋白CD36（GPIV）在剪切速率为800 s-1的流动全血中对血小板在I型胶原上黏附的贡献。在一系列实验中，从洗涤过的红细胞、血浆和已与单特异性多克隆抗CD36抗体的Fab片段（50微克/毫升，30分钟，37摄氏度）孵育过的洗涤血小板制备了重组正常血液（血细胞比容0.4；血小板计数1.5×10(5)/微升）。与配对的重组对照（100%）相比，使用抗体阻断血小板的重组血液对胶原包被盖玻片的表面覆盖百分比在2分钟时为50%，5分钟时为87%，10分钟时为90%。通过将来自Naka阴性表型健康供体的全血灌注到胶原包被的盖玻片上进行了进一步研究，该供体的血小板天然缺乏CD36。在这种情况下，表面覆盖百分比在2分钟时为正常对照的55%，5分钟时为对照的76%，10分钟时为对照的72%。在两种制剂中，缺乏功能性CD36的血小板在灌注2分钟和10分钟后黏附具有统计学显著下降（P <.005），但在5分钟时没有。这些结果表明功能性CD36促进血小板与胶原的快速黏附，并且这种作用在它们相互作用的最早时间点就可见。
