Itoh N, Tsujimoto Y, Nagata S
Osaka Bioscience Institute, Furuedai, Japan.
J Immunol. 1993 Jul 15;151(2):621-7.
Fas Ag is a cell surface protein that can mediate apoptosis and belongs to the TNF receptor family. The product of protooncogene bcl-2, a membrane-associated protein, has been shown to inhibit apoptosis in various hematopoietic cells including B cells and T cells. To examine the possible interaction of the Fas Ag and bcl-2, we coexpressed human Fas Ag and bcl-2 cDNA in murine IL-3-dependent FDC-P1 cell line and murine lymphoma WR19L. FDC-P1 transformants expressing bcl-2 showed a prolonged survival to IL-3 depletion. FDC-P1 transformants expressing the Fas Ag alone were killed by anti-Fas antibody in the presence of IL-3. Overexpression of bcl-2 in FDC-P1 resulted in a partial inhibition of Fas-induced cell death. WR19L transformants expressing bcl-2 were partially resistant to the cytolytic activities of the TNF-alpha and anti-Fas antibody treatment. These results suggest that the Fas Ag and TNF receptor may share the same signaling pathway, and that bcl-2 interferes with the apoptotic process mediated by the Fas Ag and TNF receptor.
Fas抗原是一种可介导细胞凋亡的细胞表面蛋白，属于肿瘤坏死因子受体家族。原癌基因bcl - 2的产物是一种膜相关蛋白，已被证明可抑制包括B细胞和T细胞在内的各种造血细胞的凋亡。为了研究Fas抗原与bcl - 2之间可能的相互作用，我们在小鼠白细胞介素-3依赖的FDC - P1细胞系和小鼠淋巴瘤WR19L中共同表达了人Fas抗原和bcl - 2 cDNA。表达bcl - 2的FDC - P1转化体在白细胞介素-3耗尽后存活时间延长。单独表达Fas抗原的FDC - P1转化体在有白细胞介素-3存在的情况下被抗Fas抗体杀死。bcl - 2在FDC - P1中的过表达导致Fas诱导的细胞死亡受到部分抑制。表达bcl - 2的WR19L转化体对肿瘤坏死因子-α的细胞溶解活性和抗Fas抗体处理具有部分抗性。这些结果表明，Fas抗原和肿瘤坏死因子受体可能共享相同的信号通路，并且bcl - 2干扰由Fas抗原和肿瘤坏死因子受体介导的凋亡过程。
