Lund-Johansen F, Terstappen L W
Department of Pathology, Gade Institute, University of Bergen, Haukeland Hospital, Norway.
J Leukoc Biol. 1993 Jul;54(1):47-55. doi: 10.1002/jlb.54.1.47.
Individual steps of granulocyte maturation, such as lineage commitment, proliferation, maturation, and migration from the marrow to the peripheral blood, may be influenced by distinct interactions with the bone marrow stroma. To identify candidates of membrane components involved in maturational stage-specific interactions, we studied changes in the expression of cell adhesion molecules along the granulocyte maturational pathway. Three-color flow cytometric measurements were used to measure levels of cell adhesion molecules along this pathway. The alpha chains of VLA-4 (CD49d) and VLA-5, the integrin beta 1 chain (CD29), and CD31 (PECAM-1) were expressed in high density on all early myeloid cells but down-modulated during postproliferative maturation. CD44 and L-selectin were expressed on CD34+ myeloid progenitor cells and mature granulocytes but down-modulated during the intermediate stages of maturation. The granulocyte receptor for endothelial selectins, sLex, was specifically expressed by myeloid progenitor cells. sLex was down-modulated during the intermediate stages of granulocyte maturation but up-regulated again during terminal maturation. In contrast, CD67, a putative granulocyte adhesion molecule, was negative on progenitors, transiently up-regulated during the intermediate stages of maturation, and almost absent from the surface of mature granulocytes. These results show that each stage of granulocyte maturation is associated with the expression of a unique combination of cell adhesion molecules. L-selectin, CD44, and beta 1 integrins were regulated as previously described for immature lymphopoietic cells and may therefore play general roles in the compartmentalization and development of leukocytes. In contrast, sLex and CD67 were specifically expressed by myeloid cells and could be specifically important for compartmentalization of distinct phases of granulocyte maturation.
粒细胞成熟的各个步骤，如谱系定向、增殖、成熟以及从骨髓迁移至外周血，可能受到与骨髓基质不同相互作用的影响。为了确定参与成熟阶段特异性相互作用的膜成分候选物，我们研究了沿粒细胞成熟途径的细胞黏附分子表达变化。采用三色流式细胞术测量沿此途径的细胞黏附分子水平。VLA-4（CD49d）和VLA-5的α链、整合素β1链（CD29）以及CD31（PECAM-1）在所有早期髓系细胞上高密度表达，但在增殖后成熟过程中下调。CD44和L-选择素在CD34+髓系祖细胞和成熟粒细胞上表达，但在成熟的中间阶段下调。内皮选择素的粒细胞受体sLex由髓系祖细胞特异性表达。sLex在粒细胞成熟的中间阶段下调，但在终末成熟阶段再次上调。相反，假定的粒细胞黏附分子CD67在祖细胞上呈阴性，在成熟的中间阶段短暂上调，在成熟粒细胞表面几乎不存在。这些结果表明，粒细胞成熟的每个阶段都与细胞黏附分子的独特组合表达相关。L-选择素、CD44和β1整合素如先前针对未成熟淋巴细胞所描述的那样受到调节，因此可能在白细胞的分隔和发育中发挥一般作用。相比之下，sLex和CD67由髓系细胞特异性表达，可能对粒细胞成熟不同阶段的分隔特别重要。
