Ferré S, Artigas F
Department of Neurochemistry, C.S.I.C., Barcelona, Spain.
J Neurochem. 1993 Aug;61(2):772-5. doi: 10.1111/j.1471-4159.1993.tb02187.x.
Morphological and pharmacological data suggest the existence of a reciprocal interaction between the mesencephalic dopamine (DA) system and the serotonin (5-HT) system originating in the dorsal raphe nucleus (DRN). In the present work, a DA D2 receptor-mediated regulation of 5-HT extracellular concentrations in the DRN is described, by using brain microdialysis in freely moving rats. Local infusion of the nonselective DA agonist apomorphine produced a dose-dependent increase in the extracellular concentration of 5-HT in the DRN, which was prevented by previous infusion of the specific D2 antagonist raclopride but not of the D1 antagonist SCH-23390. Furthermore, local infusion of the selective D2 agonist LY-171,555 increased extracellular 5-HT levels in the DRN, and this effect was also prevented by the previous infusion of raclopride. It is postulated that DA, either from projections from the substantia nigra or the ventral tegmental area or from the DA-containing neurons of the DRN, may increase 5-HT release in the DRN, which, through autoreceptor stimulation, can profoundly influence the activity of ascending serotoninergic neurons.
形态学和药理学数据表明，中脑多巴胺（DA）系统与起源于中缝背核（DRN）的5-羟色胺（5-HT）系统之间存在相互作用。在本研究中，通过对自由活动大鼠进行脑微透析，描述了DA D2受体介导的对DRN中5-HT细胞外浓度的调节。局部注射非选择性DA激动剂阿扑吗啡可使DRN中5-HT的细胞外浓度呈剂量依赖性增加，预先注射特异性D2拮抗剂雷氯必利可阻止这种增加，但预先注射D1拮抗剂SCH-23390则不能。此外，局部注射选择性D2激动剂LY-171,555可增加DRN中细胞外5-HT水平，预先注射雷氯必利也可阻止这种作用。据推测，来自黑质或腹侧被盖区投射的DA或来自DRN中含DA神经元的DA，可能会增加DRN中5-HT的释放，通过自身受体刺激，这会深刻影响5-羟色胺能上行神经元的活性。
