Landegren U
Department of Medical Genetics, Uppsala Biomedical Center, Sweden.
Trends Genet. 1993 Jun;9(6):199-204. doi: 10.1016/0168-9525(93)90119-3.
Protocols for in vitro amplification of nucleic acids are proliferating and there are now several methods that will contribute both to genetic research and to the diagnosis of a wide range of diseases. Here, I present the working principles of some of these molecular machines for amplifying DNA or RNA and discuss the lines along which new methods of amplifying nucleic acids may be developed.
核酸体外扩增的方法不断涌现，目前已有多种方法可应用于基因研究和多种疾病的诊断。在此，我将介绍其中一些用于扩增DNA或RNA的分子机制的工作原理，并探讨开发核酸扩增新方法的可能方向。
