大鼠磷脂酶C-β4（磷脂酶C的一个新成员）编码cDNA的克隆

Cloning of cDNA encoding rat phospholipase C-beta 4, a new member of the phospholipase C.

作者信息

Kim M J, Bahk Y Y, Min D S, Lee S J, Ryu S H, Suh P G

机构信息

Department of Life Science, Pohang University of Science and Technology, South Korea.

出版信息

Biochem Biophys Res Commun. 1993 Jul 30;194(2):706-12. doi: 10.1006/bbrc.1993.1879.

DOI:10.1006/bbrc.1993.1879

PMID:7688223

Abstract

Phospholipase C-beta 4(PLC-beta 4), a new member of phospholipase C isozyme, was purified from bovine cerebellum. The cDNA encoding rat PLC-beta 4 has been cloned from a cDNA library prepared from rat brain. The predicted open reading frame encodes a protein of 1,176 amino acids with a calculated molecular weight of 134,552. The deduced amino acid sequence exhibits 39, 36, and 36% identity with the sequences of rat PLC-beta 1, human PLC-beta 2, and rat PLC-beta 3, respectively. The amino acid sequence of PLC-beta 4, especially, shows higher identity (50%) with norpA PLC sequence from Drosophila melanogaster than those of other PLC-beta subtypes, suggesting that the PLC-beta 4 might be a mammalian PLC equivalent of norpA PLC implicated in photosignal transduction in Drosophila.

摘要

磷脂酶C-β4（PLC-β4）是磷脂酶C同工酶的一个新成员，它是从小牛小脑提纯出来的。编码大鼠PLC-β4的cDNA已从大鼠脑制备的cDNA文库中克隆出来。预测的开放阅读框编码一个由1176个氨基酸组成的蛋白质，计算分子量为134552。推导的氨基酸序列分别与大鼠PLC-β1、人PLC-β2和大鼠PLC-β3的序列具有39%、36%和36%的同一性。特别是，PLC-β4的氨基酸序列与黑腹果蝇的norpA PLC序列具有更高的同一性（50%），高于其他PLC-β亚型，这表明PLC-β4可能是果蝇中参与光信号转导的norpA PLC的哺乳动物等同物。