人肌肉乙酰胆碱受体的一级结构。γ和ε亚基的cDNA克隆。

Primary structure of the human muscle acetylcholine receptor. cDNA cloning of the gamma and epsilon subunits.

作者信息

Beeson D, Brydson M, Betty M, Jeremiah S, Povey S, Vincent A, Newsom-Davis J

机构信息

Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, England.

出版信息

Eur J Biochem. 1993 Jul 15;215(2):229-38. doi: 10.1111/j.1432-1033.1993.tb18027.x.

DOI:10.1111/j.1432-1033.1993.tb18027.x

PMID:7688301

Abstract

cDNA sequences encompassing the full coding region for the human muscle acetylcholine receptor (AChR) epsilon and gamma subunits have been isolated. The deduced amino-acid sequences indicate that the mature epsilon subunit contains 473 amino acids and is preceded by a 20-amino-acid signal peptide. As predicted from genomic clones, the gamma subunit contains 495 amino acids preceded by a 22-amino-acid signal peptide. In common with the human alpha, beta, gamma and delta subunits the epsilon subunit is highly conserved between mammalian species. The epsilon subunit gene is not closely linked to the gamma and delta subunits on chromosome 2 but rather is located with the beta subunit on chromosome 17. Expression of the alpha-, beta-, gamma-, delta- and epsilon-subunit cRNAs in rabbit-reticulocyte lysates followed by analysis on SDS/PAGE show glycosylated proteins with apparent molecular masses of 44-60 kDa.

摘要

包含人类肌肉乙酰胆碱受体（AChR）ε和γ亚基完整编码区的cDNA序列已被分离出来。推导的氨基酸序列表明，成熟的ε亚基含有473个氨基酸，前面有一个20个氨基酸的信号肽。正如从基因组克隆中预测的那样，γ亚基含有495个氨基酸，前面有一个22个氨基酸的信号肽。与人类α、β、γ和δ亚基一样，ε亚基在哺乳动物物种之间高度保守。ε亚基基因与2号染色体上的γ和δ亚基没有紧密连锁，而是与β亚基位于17号染色体上。α、β、γ、δ和ε亚基cRNA在兔网织红细胞裂解物中表达，随后在SDS/PAGE上进行分析，结果显示糖基化蛋白的表观分子量为44 - 60 kDa。