Wray L V, Fisher S H
Department of Microbiology, Boston University School of Medicine, MA 02118.
Gene. 1993 Aug 16;130(1):145-50. doi: 10.1016/0378-1119(93)90359-b.
The Streptomyces coelicolor glnR gene positively regulates the transcription of the glutamine synthetase-encoding glnA gene. The nucleotide sequence of a 1682-bp DNA segment containing glnR was determined. The deduced amino acid sequence of the GlnR protein was found to be similar to the sequence of several bacterial response regulators that are known to function as transcriptional activators. Primer extension analysis of glnR mRNA identified three transcriptional start points (tsp) upstream from the glnR coding sequence.
天蓝色链霉菌的glnR基因正向调控编码谷氨酰胺合成酶的glnA基因的转录。测定了包含glnR的1682 bp DNA片段的核苷酸序列。发现GlnR蛋白推导的氨基酸序列与已知作为转录激活因子发挥作用的几种细菌应答调节因子的序列相似。对glnR mRNA进行引物延伸分析,确定了glnR编码序列上游的三个转录起始点(tsp)。