Neuberger T J, De Vries G H
Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
J Neurocytol. 1993 Jun;22(6):436-48. doi: 10.1007/BF01181564.
The distribution of acidic and basic fibroblast growth factor in co-cultures of dorsal root ganglion neurons and Schwann cells was examined as a function of time in culture. After two days in vitro, the cytoplasm of the neuronal cell bodies demonstrated both acidic and basic FGF immunoreactivity, whereas the cytoplasm of the neurites was not immunoreactive. Schwann cells, in contrast, exhibited both acidic and basic fibroblast growth factor cytoplasmic immunoreactivity. After two days in culture, immunoreactivity was not detected on the plasma membrane surface of either the neurons or the Schwann cells. By 10 days in vitro, fibroblast growth factor immunoreactivity was observed in the cytoplasm of the most proximal portion of some, but not all, neurites but was unchanged in Schwann cells. At 20 days in vitro, immunoreactivity was still restricted to the intracellular compartment of both Schwann cells and neurons. Acidic fibroblast growth factor was primarily localized to the cytoplasm of Schwann cells, neuron cell bodies and along the entire length of the neurites. In contrast, basic fibroblast growth factor was predominantly localized to the nuclei of Schwann cells and small to medium size neurons. In many cases, the nucleolar region demonstrated the most intense basic fibroblast growth factor. The cytoplasm of the neurites was also immunoreactive for basic fibroblast growth factor. At 30 days in vitro the intracellular distribution of fibroblast growth factor immunoreactivity was similar to that observed at 20 days. However, both acidic and basic fibroblast growth factor were detected on the surface of the neurites. In contrast, no fibroblast growth factor immunoreactivity was detected at the Schwann cell surface at any time point examined. The distribution of fibroblast growth factor in Schwann cells cultured by themselves was similar to that of Schwann cells co-cultured with neurons after 20 days in vitro. Both Schwann cells and dorsal root ganglia exhibited increased fibroblast growth factor immunoreactivity with increased time in culture and an increased expression of basic fibroblast growth factor in the nucleus. Of particular interest was the appearance of fibroblast growth factor on the surface of neurites after 30 days in vitro where it could function to modulate neuron-glial cell interactions.
研究了背根神经节神经元与施万细胞共培养物中酸性和碱性成纤维细胞生长因子的分布随培养时间的变化。体外培养两天后，神经元胞体的细胞质显示出酸性和碱性FGF免疫反应性，而神经突的细胞质无免疫反应性。相比之下，施万细胞在细胞质中同时表现出酸性和碱性成纤维细胞生长因子免疫反应性。培养两天后，在神经元或施万细胞的质膜表面均未检测到免疫反应性。体外培养10天时，在一些（但不是全部）神经突最近端部分的细胞质中观察到成纤维细胞生长因子免疫反应性，而施万细胞中的情况未发生变化。体外培养20天时，免疫反应性仍局限于施万细胞和神经元的细胞内区室。酸性成纤维细胞生长因子主要定位于施万细胞、神经元胞体的细胞质以及神经突的全长。相比之下，碱性成纤维细胞生长因子主要定位于施万细胞和中小尺寸神经元的细胞核。在许多情况下，核仁区域显示出最强的碱性成纤维细胞生长因子。神经突的细胞质对碱性成纤维细胞生长因子也有免疫反应性。体外培养30天时，成纤维细胞生长因子免疫反应性的细胞内分布与20天时观察到的相似。然而，在神经突表面检测到了酸性和碱性成纤维细胞生长因子。相比之下，在任何检测的时间点，施万细胞表面均未检测到成纤维细胞生长因子免疫反应性。体外培养20天后，单独培养的施万细胞中生长因子的分布与与神经元共培养的施万细胞相似。随着培养时间的延长，施万细胞和背根神经节的成纤维细胞生长因子免疫反应性均增加，且细胞核中碱性成纤维细胞生长因子的表达增加。特别令人感兴趣的是，体外培养30天后神经突表面出现了成纤维细胞生长因子，它可能在调节神经元 - 神经胶质细胞相互作用中发挥作用。
