Hamano T, Kaji R, Diaz A F, Kohara N, Takamatsu N, Uchiyama T, Shibasaki H, Kimura J
Department of Neurology, Faculty of Medicine, Kyoto University, Japan.
Electroencephalogr Clin Neurophysiol. 1993 Aug;89(4):278-86. doi: 10.1016/0168-5597(93)90107-z.
To evaluate sensory response to natural stimuli, we developed a method to record sensory nerve action potentials evoked by high-frequency vibratory stimulation. Trains of 250 Hz sinusoidal indentations with a duration of 100 msec were applied to the finger tip at a rate of 3 Hz. The responses were obtained with surface electrodes placed over the median and ulnar nerves at the wrist. Averaged wave forms consisted of two components: an initial diphasic or triphasic potential and the following low amplitude trains of discharges phase-locked to vibratory stimuli. Anesthesia of the skin at the stimulation site or tourniquet-induced ischemia of the limb eventually diminished both components. Neither of them therefore represented artifacts from electrode movement or electromagnetic field intrinsic to the stimulator. Various kinds of mechanoreceptors may contribute to the first component. Pacinian corpuscles probably give rise to the second component as the only skin receptors that can respond to high-frequency vibratory stimuli of 250 Hz. This method helps examine neural coding of the receptor and the peripheral nerve fiber mediating vibration sense.
为了评估对自然刺激的感觉反应，我们开发了一种方法来记录高频振动刺激诱发的感觉神经动作电位。以3Hz的频率向指尖施加持续时间为100毫秒的250Hz正弦压痕序列。通过置于手腕处正中神经和尺神经上方的表面电极获得反应。平均波形由两个成分组成：一个初始的双相或三相电位以及随后与振动刺激锁相的低振幅放电序列。刺激部位皮肤的麻醉或止血带引起的肢体缺血最终会使这两个成分都减弱。因此，它们都不代表电极移动或刺激器固有的电磁场产生的伪迹。各种机械感受器可能对第一个成分有贡献。环层小体可能是产生第二个成分的原因，因为它是唯一能对250Hz高频振动刺激做出反应的皮肤感受器。这种方法有助于研究介导振动觉的感受器和周围神经纤维的神经编码。
